Bin2 point mutation

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August undefined, 2024

WebDec 9, 2024 · Key message Auxin can alter the fertility of bin2-1 plants and depends on the expression of SHY2. Abstract Brassinosteroids (BRs) play important roles in plant growth and developmental processes. By systematically evaluating the phenotypes of BR biosynthesis and BR signaling mutants, researchers have reported that BRs positively … WebJan 1, 2012 · BIN2 encodes a GSK3 with a catalytic domain sharing ∼70% identity with animal GSK3β 17, 18, 19, 20 (Figure 1). Gain-of-function bin2 point mutations and …

A secreted peptide acts on BIN2-mediated …

WebDec 29, 2024 · To further determine the impact of the mutation on the BR signaling outputs, we expressed native promoter-driven genomic BIN2 and the corresponding mutated variants in Col-0. These mutations led to a larger plant stature (Figs 5d, S8) and the accumulation of more unphosphorylated BES1 compared with the plants expressing wild-type BIN2 (Fig. … http://sourcedb.ib.cas.cn/cn/ibthesis/201003/P020100329363315667749.pdf how many syllables in arrived

BIN2, a New Brassinosteroid-Insensitive Locus in Arabidopsis Plant Phy…

WebJan 1, 2012 · The kinase-dead mutation bin2 (K69R) that suppresses gain-of-function mutations is highlighted in light green. Homologous residues to those identified by mutation in At SK32 [48] are marked with white arrowheads. The serine 9 residue specific to GSK3β and essential to the regulation of its inhibition is marked with a black arrowhead. WebThe downstream signal transduction involves a gene isolated by forward genetic studies, BRASSINOSTEROID-INSENSITIVE 2 (BIN2). Dominant mutations in bin2 result in … WebNov 1, 2002 · As expected, the bin2-1-mutated BIN2 protein showed a higher kinase activity toward BES1 or BZR1 than the wild-type BIN2, whereas no phosphorylation by the wild … how many syllables in balloon

Genetic analysis of T-DNA insertional mutations of BIN2 …

Adenosine monophosphate deaminase modulates BIN2 activity …

WebBIN2_ENST00000544402 - Explore an overview of BIN2_ENST00000544402, with a histogram displaying coding mutations, full tabulated details of all associated variants, tissue distribution and any drug resistance data. WebAug 1, 2016 · BIN2 acts as a novel downstream effector of TOR signaling to negatively regulate photoautotrophic growth. To identify the direct partner of AtS6K2, yeast two-hybrid screening was performed to identify the putative proteins that bind to the given … New Phytologist Foundation - Wiley Online Library how did you frame the challenge at the timeWebSep 24, 2024 · TOR is required for BL-mediated hypocotyl growth. Previous studies suggested that the TOR and BR signaling pathways are coordinated, as TOR regulates the BR signaling components BIN2 [Citation 32] and BZR1 [Citation 33].BRs regulate the expression of thousands of genes through transcription factors including BES1 and … how did you find this activity

"Webdet2-1 and BR-insensitive mutant bin2-1 are hypersensitive to salinity stress during seed germination and/or the seed-ling growth stage. Moreover, the hypersensitivity corre … " - Bin2 point mutation

Bin2 point mutation

BIN2, a new brassinosteroid-insensitive locus in Arabidopsis

WebMar 3, 2024 · A point mutation in BnaC01.BIN2 leads to a dwarf phenotype, and heterozygosity in the BnDF4 allele leads to short stature without marked differences in … WebJan 30, 2024 · For generation the mutant forms of BIN2 and CO, their coding sequences were first cloned into the pQBV3 vector, and then the point mutations were introduced by the specifically designed primers as previously described ( Zheng et al., 2004 ), following the PCR amplification program: preheating at 94°C for 3 min, 16 cycles of 94°C for 1 min, …

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WebJul 11, 2002 · BIN2 encodes a member of the glycogen synthase kinase-3 (GSK3)-like kinases ( 14, 15 ). The increased kinase activity by the semidominant bin2–1 mutation … WebBIN2_ENST00000604560 - Explore an overview of BIN2_ENST00000604560, with a histogram displaying coding mutations, full tabulated details of all associated variants, tissue distribution and any drug resistance data.

WebGenetic analysis of T-DNA insertional mutations of BIN2 and its two closest homologs. A, Schematic representation of T-DNA insertions in the BIL1 and BIL2 genes. Black boxes represent... WebNov 9, 2024 · The gain-of-function mutant bin2-1 contains an E263K mutation in the catalytic domain of BIN2 and is semidominant, with the homozygous mutant displaying severe growth defects (Li et al., 2001 ). The heterozygous bin2-1 mutant ( bin2-1 HT) was hypersensitive to salt stress ( Figures 2 C and 2E).

WebBIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling GLYCOGEN SYNTHASE KINASE3 (GSK3) is a highly conserved serine/threonine kinase involved in a variety … WebBIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling GLYCOGEN SYNTHASE KINASE3 (GSK3) is a highly …

WebJan 30, 2024 · For generation the mutant forms of BIN2 and CO, their coding sequences were first cloned into the pQBV3 vector, and then the point mutations were introduced …

WebAug 25, 2016 · In the BR signaling pathway, the negative regulator BIN2 is one of 10 glycogen synthase kinase 3 (GSK3)-like kinases (GSK3s) encoded in the A. … how did you find out you had celiac diseaseWebBIN2 (brassinosteroid-insensitive 2) kinase, a GSK3-like kinase functioning as a negative regulator in BR signaling, directly phosphorylated RAPTOR1B (regulatory-associated protein of TOR 1B), a ... how did you find out 意味WebDec 22, 2013 · All point mutants of BIN2 (bin2-1/BIN2 E263K, BIN2 K69R) and ARF7 (ARF7 S698/707A) were generated using the QuikChange Site-Directed Mutagenesis kit (Stratagene) according to the manufacturer’s ... how many syllables in bandageWebMar 27, 2024 · HY5 L137D mutation reduces BIN2 activity. To understand which amino acid residues of HY5 were important for its interaction with BIN2, we screened BIN2-interacting sites in the HY5 LZ region. how did you get ahold of a copy of the bugleWebJul 25, 2024 · Our aim was to find unknown BIN2 regulators via a forward genetic screen for bikinin-resistant mutants in Arabidopsis. Findings: We discovered that a mutation in the ADENOSINE MONOPHOSPHATE DEAMINASE ( AMPD )/ EMBRYONIC FACTOR1 ( FAC1) gene reduces the sensitivity of Arabidopsis seedlings to both bikinin and BRs. how did you generate waves in a slinky coil how did you find the cursed iceWebGenetic analysis of the bin2 (BR-INSENSITIVE 2) mutant indicated that the BR-insensitive dwarf phenotype was due to a semidominant mutation in the BIN2 gene that mapped … how many syllables in branch